1. Return the free nucleotides to approximately 50 and SETUP the simulation, press GO/STOP.
2. Unwind the DNA, separate the stands, attach the polymerase to the TOP strand ONLY. Observe the top strand of DNA, now.
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3. After the top strand is finished, turn SUBSTITUTIONS to ON and attach polymerase to the bottom strand. Observe replication of the bottom DNA strand.
4. Press GO/STOP to end the simulation.
5. Check the readout boxes for your top and bottom DNA strands (as in the picture below)
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